Induction by hypoxia of heterologous-protein production with the K1PDC1 promoter in yeasts

The possibility of a practical application of the hypoxic regulation of the K1PDC1 promoter is investigated by cloning the whole promoter sequence upstream to several heterologous genes coding for proteins widely heterogeneous in their characteristics is presented. Based on the results it is proposed that the Zygosaccharomyces bailii yeast could be used as K1PDC1 promoter-based expression vectors to achieve heterologous production in a simple and cheap procedure.