Back to Search Start Over

Centimorgan-range one-step mapping of fertility traits using interspecific recombinant congenic mice

Authors :
L'Hote, David
Serres, Catherine
Laissue, Paul
Oulmouden, Ahmad
Rogel-Gaillard, Claire
Montagutelli, Xavier
Vaiman, Daniel
Source :
Genetics. July, 2007, Vol. 176 Issue 3, p1907, 15 p.
Publication Year :
2007

Abstract

In mammals, male fertility is a quantitative feature determined by numerous genes. Until now, several wide chromosomal regions involved in fertility have been defined by genetic mapping approaches; unfortunately, the underlying genes are very difficult to identify. Here, 53 interspecific recombinant congenic mouse strains (IRCSs) bearing 1-2% SEG/Pas (Mus spretus) genomic fragments disseminated in a C57B1/ 6J (Mus domesticus) background were used to systematically analyze male fertility parameters. One of the most prominent advantages of this model is the possibility of analyzing stable phenotypes in living animals. Here, we demonstrate the possibility in one-step fine mapping for several fertility traits. Focusing on strains harboring a unique spretus fragment, we could unambiguously localize two testis and one prostate weight-regulating QTL (Ltw1, Ltw2, and Lpw1), four QTL controlling the sperm nucleus shape (Sh1, Sh2, Sh3, and Sh4), and one QTL influencing sperm smwival (Dss1). In several cases, the spretus DNA fragment was small enough to propose sound candidates. For instance, Spatal, Capza, and TubaTare very strong candidates for influencing the shape of the sperm head. Identifying new genes implied in mammalian fertility pathways is a necessary prerequisite for clarifying their molecular grounds and for proposing diagnostic tools for masculine infertilities.

Details

Language :
English
ISSN :
00166731
Volume :
176
Issue :
3
Database :
Gale General OneFile
Journal :
Genetics
Publication Type :
Academic Journal
Accession number :
edsgcl.167586266