Mutagenesis of lysine 62, asparagine 64, and conserved region 1 reduces the activity of human ecto-ATPase (NTPDase 2)

The mutagenesis of various complexes, including lysine 62, asparagine 64 and conserved region 1 is found to highly reduce the activity of the human ecto-ATPase (NTPDase) that contains several conserved motifs and lysine and arginine residues. The results show that most of the residues in the complex are essential for protein expression and that the substitution of such acidic residues by alanine leads to a decreased divalent ion binding.