Substitutions in the N-Glycan core as regulators of biorecognition: The case of core-fucose and bisecting GlcNAc moieties

Chemoenzymatic synthesis of the respective galactosylated and alpha2,3/6-sialylated N-glycans is performed to address the issue whether core fucosylation and the bisecting N-acetylglucosamine residue modulate ligand properties of complex-type biantennary N-glycans. The study results help in devising new, non-natural modifications to realize the full potential of glycoengineering for diagnostic and therapeutic purposes.