Novel nonlinear contrast improves deep-tissue microscopy: femtosecond-laser pulse and pulse-train shaping allow detection of new nonlinear effects with modest powers, making many new biomarkers accessible and permitting deeper tissue imaging than conventional microscopy

For centuries, the optical microscope has been the premier workhorse tool for quantitative understanding of cellular and tissue structure at the submicron level. But conventional microscopy has some important limitations; [...]