Synthesis and DNA cleavage activity of artificial receptor 1,4,7-triazacyclononane containing guanidinoethyl and hydroxyethyl side arms

The design and synthesis of a novel phosphodiester receptor 1-(2-guanidinoethyl)-4-(2-hdyroxyethyl)1-1,4,7-triazacyclononane hydrochloride and the studies of its DNA cleavage activity is reported. Kinetic data of DNA cleavage promoted by 1-(2-guanidinoethyl)-4-(2-hdyroxyethyl)1-1,4,7-triazacyclononane hydrochloride fit to a Michaelis-Menten-type equation with [K.sub.max] of 0.160 [h.sup.-1] giving [10.sup.7]-fold rate acceleration over uncatalyzed DNA.