A novel function for the N-terminal nucleophile hydrolase fold demonstrated by the structure of an archaeal inosine monophosphate cyclohydrolase

Authors :: You-Na Kang
Tran, Anh
White, Robert H.
Ealick, Steven E.
Source :: Biochemistry. May 1, 2007, Vol. 46 Issue 17, 5050-5062
Publication Year :: 2007
Abstract: The crystal structure of Methanothermobacter thermoautotrophicus inosine 5'-monophosphate (IMP) cyclohydrolase in complex with either IMP or 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) at 2.0 and 2.6 Angstrom resolution, respectively, is reported. A mechanism is proposed for the MthPurO-catalyzed cyclohydrolase reaction and is compared to that of cyclohydrolase (PurH) and of green fluorescent protein (GFP).

Subjects :: Adenylic acid -- Structure
Adenylic acid -- Research
Bacterial proteins -- Genetic aspects
Bacterial proteins -- Research
Gene expression -- Analysis
Biological sciences
Chemistry

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