Assembly of the prothrombinase complex on lipid vesicles depends on the stereochemical configuration of the polar headgroup of phosphatidylserine

Ellipsometric analysis helps study the role of the stereochemical arrangement of phosphatidylserine in the attachment of prothrombinase complex to phospholipid surface. The attachment reaction requires prothrombinase complexes consisting of Xa and Va factors as catalysts. The Xa and Va factors are arranged on phospholipid surface by calcium-mediated reactions with headgroups having polar lipids. The arrangement of prothrombinase complex is enhanced by the membranes having L-alpha-glycarophosphoryl-D-serine (POS).