Capillary zone electrophoresis for gliadin separation: applications in a spelt breeding program

Byline: Tilman J. Schober (1), Manfred Kuhn (2) Keywords: Capillary electrophoresis; Spelt; Triticum aestivum ssp. spelta; Wheat; Gliadin; Cultivars; Breeding programs Abstract: Gliadins of 27 European spelt [Triticum aestivum ssp. spelta (L.) Thell.] cultivars (cvs) were separated by capillary zone electrophoresis (CZE). A great amount of diversity was found within the CZE gliadin patterns of these cvs however, only 15 had unique patterns, whereas 2 triples and 3 pairs were identical, i.e., differences were within the uncertainty of the method and environmental effects. Two of these identical pairs were attributed to mislabeling: affected were one of the two Swiss spelt cvs 'Ostar' and 'Sertel', and the German spelt cv 'Steiners Roter Tiroler'. The German cv 'Franckenkorn' showed considerable similarity with the Belgian cv 'Rouquin'. CZE gliadin patterns of 12 modern German bread wheat cvs were distinctly different from those of spelt cvs selected from old land varieties like the Swiss cv 'Oberkulmer Rotkorn'. In the gliadin patterns of 11 spelt cvs, bread wheat elements were found. 7 of the 24 cvs not mislabeled are known to be progeny of wheat/spelt crosses, 4 cvs are described as pure spelt, and the pedigrees of 13 are unknown. Wheat elements were spotted in only 4 of the 7 wheat/spelt crosses and in none of the 4 pure spelt cvs. The results suggest that crossing spelt with modern wheat may be, but is not necessarily, reflected in the gliadin pattern. Author Affiliation: (1) Department of Food and Nutritional Sciences (Food Technology), University College Cork, National University of Ireland, College Road, Cork, Ireland (2) Institute 150 (Food Technology), Section Cereal Technology, University of Hohenheim, Garbenstrasse, 25, 70599, Stuttgart-Hohenheim, Germany Article History: Received Date: 13/02/2003 Online Date: 22/07/2003