Experimental study of the embryogenesis of gastrointestinal duplication and enteric cyst

Byline: Takaki Emura (1), Kohei Hashizume (1), Makoto Asashima (2) Keywords: Split notochord syndrome Gastrointestinal duplication Enteric cyst Split cord malformation Embryogenesis Abstract: The theory of gastrointestinal duplication and enteric cyst embryogenesis was verified by examining the developmental process of this experimentally induced anomaly. In Cynopus pyrrhogaster (amphibian) embryos (stage 18), the dorsal midline structures (including the neural plate and notochord) were split regionally to induce partial separation of the notochord and gut anlage endoderm herniation between the split elements of the notochord. Following this procedure, the embryonic development was traced morphologically and histologically. Control embryos were cultured without the procedure. Following the incubation and breeding period, gastrointestinal duplication and enteric cysts were observed with vertebral anomaly, spina bifida, split cord malformation and subcutaneous manifestations in the mature animals. The combination of anomalies that was observed in these experimental animals is consistent with that found in 'split notochord syndrome.' No abnormal morphology or histology was observed in the control group. The embryogenetic theory of gastrointestinal duplication and enteric cysts was thus verified by simulating the partial separation of the notochord, which induced split notochord syndrome in laboratory animals. The results indicate that gastrointestinal duplication and enteric cysts may arise through a process of herniation of the gut anlage endoderm between split elements of the notochord. Author Affiliation: (1) Department of Pediatric Surgery, Graduate School of Medicine, University of Tokyo, Tokyo, Japan (2) Department of Life Science, CREST Project, Graduate School of Arts and Sciences, University of Tokyo, Tokyo, Japan Article note: Electronic Publication