X-ray crystal structure of gamma-chymotrypsin in hexane

Analysis of crystal structures of gamma-chymotrypsin in hexane-soaked crystals and aqueous environment at 2.2 angstroms resolution reveals that the native structure has 97 water molecules while the hexane-soaked crystal has 130 water molecules and seven hexane molecules. The thermal factors for both water molecules and protein atoms in the native structure are higher than those in the hexane structure.