Structural confirmation of a bent and open model for the initiation complex of T7 RNA polymerase

The research makes use of a structure model, in addition to the various energy transfer distance measurements to study the T7 RNA polymerase, which causes bending of its promoter DNA on binding by various proteins and other complexes. The analysis proves the existence of a bent and an open model for the initiation complex of T7 and also shows that in spite of the massive changes, the DNA of the active site is very much identical in initiation as well as elongation complexes.