Constitutive expression of the virulence genes improves the efficiency of plant transformation by Agrobacterium

Authors :: Hansen, Genevieve
Das, Anath
Chilton, Mary-Dell
Source :: Proceedings of the National Academy of Sciences of the United States. August 2, 1994, Vol. 91 Issue 16, p7603, 5 p.
Publication Year :: 1994
Abstract: Agrobacterium tumefaciens incorporated with VirGN54D plasmids contain constitutive virulence (Vir) gene expressions and enhance the transfer properties of transferred DNA (T-DNA). Beta-glucuronidase (GUS) reporter gene used to determine post-transfer T-DNA expression reveals an increase in GUS-expressing foci. Wild-type VirG gene transcripts enhance T-DNA expression in tobacco but not in maize.

Subjects :: Agrobacterium tumefaciens -- Genetic aspects
Gene expression -- Analysis
Genetic transcription -- Analysis
Science and technology

ISSN :: 00278424
Volume :: 91
Issue :: 16
Database :: Gale General OneFile
Journal :: Proceedings of the National Academy of Sciences of the United States
Publication Type :: Academic Journal
Accession number :: edsgcl.16077969

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