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Use of transcriptional fusions to monitor gene expression: a cautionary tale

Authors :
Forsberg, Ake J.
Pavitt, Graham D.
Higgins, Christopher F.
Source :
Journal of Bacteriology. April, 1994, Vol. 176 Issue 7-8, p2128, 5 p.
Publication Year :
1994

Abstract

Gene expressions and promoter functions are analyzed by using transcriptional fusion in genes. Reporter genes increase promoter activity. The luxA gene inhibits or activates transcription of promoters due to the curved DNA fragment in 5' sequence of the gene. The DNA sequence is eliminated by inducing multiple mutations. LuxAB is a single reporter gene used in promoter activity.

Details

ISSN :
00219193
Volume :
176
Issue :
7-8
Database :
Gale General OneFile
Journal :
Journal of Bacteriology
Publication Type :
Academic Journal
Accession number :
edsgcl.15489441