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Liver-directed gene therapy: quantitative evaluation of promoter elements by using in vivo retroviral transduction

Authors :
Rettinger, Steven D.
Kennedy, Susan C.
Wu, Xiaoyun
Saylors, Robert L.
Hafenrichter, Daniel G.
Flye, M. Wayne
Ponder, Katherine Parker
Source :
Proceedings of the National Academy of Sciences of the United States. Feb 15, 1994, Vol. 91 Issue 4, p1460, 5 p.
Publication Year :
1994

Abstract

A liver-directed gene therapy is used with concentrated retroviral preparations to obtain transduction of up to 15% of hepatocytes as established by a quantitative PCR assay. In vivo comparisons of gene regulatory elements are possible with these quantitative assays. Transduced gene expression will continue to prevent difficulties.

Details

ISSN :
00278424
Volume :
91
Issue :
4
Database :
Gale General OneFile
Journal :
Proceedings of the National Academy of Sciences of the United States
Publication Type :
Academic Journal
Accession number :
edsgcl.15303663