Effects of arginine vasopressin and atriopeptin on glial cell volume measured as 3-MG space

Cultured astroglial cells obtained from newborn rats are used to determine whether arginine vasopressin and atriopeptin control brain cell volume. The regulators are assumed to be peptide hormones prepared and secreted inside the brain. A clear serum-free medium such as the 3-O-methylglucose (3-MG) space is used as a channel to calculate the cell water content, considered to be the volume. The calculation of the 3-MG space is independent of glucose, though the glucose carrier is required by 3-MG and glucose.