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Characterization of human saposins by NMR spectroscopy

Authors :
John, Michael
Wendeler, Michaela
Heller, Markus
Sandhoff, Konrad
Kessler, Horst
Source :
Biochemistry. April 25, 2006, Vol. 45 Issue 16, p5206, 11 p.
Publication Year :
2006

Abstract

The solution NMR spectroscopy is employed to characterize all four (super 15)N-labeled human saposins at both neutral and acidic pH. The results reveal that pH 4.0, where all saposins are highly unstable, Sap-C undergoes a transition to a specific dimeric state, which might resemble the structure found in both Sap-C in a detergent environment and crystals of Sap-B.

Details

Language :
English
ISSN :
00062960
Volume :
45
Issue :
16
Database :
Gale General OneFile
Journal :
Biochemistry
Publication Type :
Academic Journal
Accession number :
edsgcl.147193153