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Virus electrodes for universal biodetection
- Source :
- Analytical Chemistry. May 15, 2006, Vol. 78 Issue 10, p3265, 6 p.
- Publication Year :
- 2006
-
Abstract
- A dense virus layer, readily tailored for recognition of essentially any biomarker, was covalently attached to a gold electrode surface through a self-assembled monolayer. The resistance of this 'virus electrode', [Z.sub.Re], measured in the frequency range from 2 to 500 kHz in a salt-based pH 7.2 buffer, increased when the phage particles selectively bound either an antibody or prostate-specific membrane antigen (PSMA), a biomarker for prostate cancer. In contrast to prior results, we show the capacitive impedence of the virus electrode, [Z.sub.Im], is both a noisier and a less sensitive indicator of this binding compared to [Z.sub.Re]. The specificity of antibody and PSMA binding, and the absence of nonspecific binding to the virus electrode, was confirmed using quartz crystal microbalance gravimetry.
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 78
- Issue :
- 10
- Database :
- Gale General OneFile
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.146892923