PNA-based reagents for the direct and site-specific synthesis of thymine dimer lesions in genomic DNA

Several biochemical, crystallographic and cellular studies are conducted to observe the direct and site-specific synthesis of thymine dimmer lesions, which is very important phenomenon to learn how DNA lesions induce mutations and how the lesions are removed from genome by the cells. The results show that the peptide nucleic acids (PNA) reagents are very useful in recognizing the targeted sequence in a large genomic DNA fragment, which can be further used to generate selectivity by the irradiation of the undesired lesion.