Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from streptomyces griseus

The fredericamycin (FDM) A, is a pentadecaketide that features two groups of peri hydroxyl tricyclic aromatic moieties associated via a distinct chiral spiro carbon center, which shows effective cytotoxicity and is fully analyzed as an anticancer drug. The fdm gene cluster was localized to 33-kb DNA segment of Steptomyces griseus ATCC 49344, and its involvement in FDM A biosynthesis was proven by gene inactivation, complementation, and heterogonous expression experiments.