Expression of the nifH gene of a Herbaspirillum endophyte in wild rice species: Daily Rhythm during the light-dark cycle

The expression of nitrogenase genes of Herbaspirillum sp. B501 associated in shoot of wild rice, Oryza officinalis, is explored by means of reverse transcription-PCR (RT-PCR) targeted at the nifH gene. The results indicate that in planta nitrogen fixation by the endophyte shows a daily rhythm determined by the plant's light environment.