Conformational changes in foot protein of the sarcoplasmic reticulum assessed by site-directed fluorescent labeling

A study was conducted on the conformational changes occurring in the foot protein of the sarcoplasmic reticulum. Biphasic activity was observed through fluorescent labeling. Based on fluorescence intensities, it was suggested that Ca2+ release modulation via ryanodine necessitates a conformational change in the neomycin-binding region of the foot protein. Ca2+ modulation of Ca2+ release was also found to involve conformational changes in the polylysine-binding region as well.