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Fragmentation of proteins in the 13- to 29-kDa mass range observed by (super 252)Cf-plasma desorption mass spectrometry

Authors :
Bunk, David M.
Macfarlane, Ronald D.
Source :
Proceedings of the National Academy of Sciences of the United States. July 1, 1992, Vol. 89 Issue 13, p6215, 5 p.
Publication Year :
1992

Abstract

Cf-plasma desorption mass spectrometry (PDMS) was used to study the fragmentation of ribonuclease A, papain and proteinase K, all of which are within the 13- to 29-kDa mass range. The results showed that the plasma desorption process could induce efficient, sequence-specific fragmentation of large, native proteins. The procedure could be used as a starting step in the sequence determination of large protein molecules.

Details

ISSN :
00278424
Volume :
89
Issue :
13
Database :
Gale General OneFile
Journal :
Proceedings of the National Academy of Sciences of the United States
Publication Type :
Academic Journal
Accession number :
edsgcl.14258036