The C2A domain of synaptotagmin exhibits a high binding affinity for copper: Implications in the formation of the multiprotein FGF release complex

Study is conducted to show that the cytoplasmic C2A domain of synaptotagmin binds to CU(super 2+) ions with high affinity. The result from isothermal calorimetry (ITC), near UV circular dischroism (CD), and absorption spectroscopy shows that four CU(super 2+) ions bind per molecule of C2A domain.