Time-resolved absorption studies of native etiolated oat phytochrome

Time-resolved absorption measurements are performed on the forward phototransformation of native oat phytochrome at 10 degrees C. Likewise, laser photolysis allows for the measurement of the transient difference absorption spectra at delay times from 100 ns to 800 ms. A global fitting algorithm, together with a singular value decomposition method, is used to evaluate the absorption changes at different wavelengths which follow the same kinetics.