Rapid, high-level expression of biologically active alpha-trichosanthin in transfected plants by an RNA viral vector

Authors :: Kumagai, M.H.
Turpen, T.H.
Weinzettl, N.
Della-Cioppa, G.
Turpen, A.M.
Donson, J.
Hilf, M.E.
Grantham, G.L.
Dawson, W.O.
Chow, T.P.
Piatak, M., Jr.
Grill, L.K.
Source :: Proceedings of the National Academy of Sciences of the United States. Jan 15, 1993, Vol. 90 Issue 2, p427, 4 p.
Publication Year :: 1993
Abstract: Trichosanthes kirilowii produces alpha-trichosanthin, a eukaryotic ribosome-inactivating protein which is currently being evaluated for its ability to inhibit human immunodeficiency virus replication. A plant viral vector that allows the expression of alpha-trichosantin in transfected plants was developed. The vector, BGC152, contains a tobacco mosaic virus promoter which directs the expression of alpha-trichosantin. Transfected Nicotiana benthamiana plants expressed alpha-trichosantin at levels of at least 2% of total soluble proteins.

Subjects :: Plant genetic engineering -- Research
Genetic vectors -- Research
Nicotiana -- Research
Trichosanthin -- Research
Plant proteins -- Genetic aspects
Science and technology

ISSN :: 00278424
Volume :: 90
Issue :: 2
Database :: Gale General OneFile
Journal :: Proceedings of the National Academy of Sciences of the United States
Publication Type :: Academic Journal
Accession number :: edsgcl.14051332

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