UV-visible spectroscopy of adsorbed cytochrome c on tin oxide electrodes

Tin oxide electrodes with an adsorbed cytochrome c monolayer were examined using ultraviolet-visible spectroscopy. The structural state and the distribution of the compound on the surface were readily observed without the need for complex procedures. The position and intensities of the ultraviolet spectra provided the information regarding the conformation and total surface coverage of the cytochrome c monolayer. In addition, ferricytochrome c remained intact after its adsorption on the electrode's surface even in the presence of applied potentials reaching 0.7 volts.