Locatioin of the hisGDCBHAFI operon on the physical map of Escherichia coli

The eight Escherichia coli enzymes involved in the synthesis of histidine are encoded by the hisGDCBHAFI operon. Based on sequence data previously established, different restriction sites were deduced and used to map the position of the operon within the Kohara physical map. The results showed that the histidine operon is located around kb 2,100, corresponding to the 44-minute region.