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Elucidating the substrate specificity and condensation domain activity of FkbP, the FK520 pipecolate-incorporating enzyme

Authors :
Gatto, Gregory J., Jr.
McLoughlin, Shaun M.
Kelleher, Neil L.
Walsh, Christopher T.
Source :
Biochemistry. April 26, 2005, Vol. 44 Issue 16, 5993-6002
Publication Year :
2005

Abstract

The expression and purification of recombinant FkbP from the FK520 biosynthetic pathway is reported. The observations suggest that the adenylation domain of FkbP serves as the primary selectivity filter for pipecolate incorporation.

Details

Language :
English
ISSN :
00062960
Volume :
44
Issue :
16
Database :
Gale General OneFile
Journal :
Biochemistry
Publication Type :
Academic Journal
Accession number :
edsgcl.136425293