Heme reduction by intramolecular electron transfer in cysteine mutant myoglobin under carbon monoxide atmosphere

Cysteine to various sites on the surface of sperm whale human myoglobin (Mb) is introduced by mutation to investigate the effects of a cysteine residue on Mb. The rate constant for heme reduction increased for higher mutant Mb concentration, whereas it did not change significantly when the CO concentration is reduced from 100% Co to 50% Co with 50% O2.