Importance of primer selection for the detection of hepatitis C virus RNA with the polymerase chain reaction assay

Authors :: Bukh, Jens
Purcell, Robert H.
Miller, Roger H.
Source :: Proceedings of the National Academy of Sciences of the United States. Jan 1, 1992, Vol. 89 Issue 1, p187, 5 p.
Publication Year :: 1992
Abstract: A cDNA polymerase chain reaction (PCR) assay for screening of hepatitis c virus (HCV) was developed using primers from conserved regions of the HCV genome. Four primer sets were compared for their efficiency in detecting HCV RNA nested in cDNA PCR assay on sera from different anti-HCV antibody positive individuals in various parts of the world. The primer sets from two highly conserved regions within the 5' noncoding region was found to be the most effective detector of HCV RNA. The results suggested the potential use of primer sets for the detection of HCV RNA in infected persons.

Subjects :: Hepatitis C virus -- Research
RNA viruses -- Research
Polymerase chain reaction -- Research
Hepatitis C -- Diagnosis
Science and technology

ISSN :: 00278424
Volume :: 89
Issue :: 1
Database :: Gale General OneFile
Journal :: Proceedings of the National Academy of Sciences of the United States
Publication Type :: Academic Journal
Accession number :: edsgcl.13595046

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