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Use of 16S rRNA gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces

Authors :
Matsuki, Takahiro
Watanabe, Koichi
Fujimoto, Junji
Takada,Toshihiko
Tanaka, Ryuichiro
Source :
Applied and Environmental Microbiology. Dec, 2004, Vol. 70 Issue 12, 7220-7228
Publication Year :
2004

Abstract

Sixteen S rRNA gene-targeted group-specific primers are designed and validated for specific detection and quantification of the Clostridium leptum subgroup and the Atopobium cluster. Examination of changes in the population and the composition of the intestinal flora reveal that the composition of the flora of each volunteer remained stable throughout the test period.

Details

Language :
English
ISSN :
00992240
Volume :
70
Issue :
12
Database :
Gale General OneFile
Journal :
Applied and Environmental Microbiology
Publication Type :
Academic Journal
Accession number :
edsgcl.133066313