Dissecting the effects of DNA polymerase and ribonuclease H inhibitor combinations on HIV-1 reverse-transcriptase activities

The impact of three mechanistically distinct reverse transcription (RT) polymerase inhibitors on ribonuclease H (RNase H) activity in vitro is analyzed by using both gel assays and a real-time FRET. The nucleoside analogue 3'-azido-3'-deoxythymidine triphosphate (AZT-TP) had no effect, whereas the pyrophosphate analogue phosphonoformate (PFA) inhibited ENase H activity in a concentration-dependent manner.