Enthalpic and entropic effects of salt and polyol osmolytes on site-specific protein-DNA association: The integrase Tn916-DNA complex

The effect of low molecular-weight compounds on the equilibrium constant K(sub A) can be used to explore the energetics and molecular mechanism of protein- DNA interactions. The complex composed of the integrase Tn916 DNA-binding domain and its target DNA duplex was used to investigate the effects of salt and the nonionic osmolytes glycerol and sorbitol on sequence-specific protein-DNA association.