Characterization of phospholipase activity of the Pseudomonas aeruginosa type III cytotoxin, ExoU

Authors :: Sato, Hiromi
Feix, Jimmy B.
Hillard, Cecilia J.
Frank, Dara W.
Source :: Journal of Bacteriology. Feb, 2005, Vol. 187 Issue 3, p1192, 4 p.
Publication Year :: 2005
Abstract: Recombinant ExoU (rExoU) and yeast extract were used to optimize an in vitro phospholipase assay as a basis for identifying the mechanism for enzyme activation and substrate specificity. Our results support a model in which a eukaryotic protein cofactor or complex facilitates the interaction of rExoU with phospholipid substrates.

Subjects :: Phospholipases -- Research
Pseudomonas aeruginosa -- Genetic aspects
Genetic research
Biological sciences

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