Effects of yo-yo diet, caloric restriction, and olestra on tissue distribution of hexachlorobenzene

Chlorinated hydrocarbons are lipophilic, toxic, and persistent in the environment and animal tissues. They enter the body in food and are stored in adipose tissue. Loss of body fat through caloric restriction mobilizes stored lipophilic xenobiotics and results in distribution to' other tissues. We have studied the reversibility of this process in mice that followed a regimen of body weight cycling. Weight gain was followed by weight loss, a second gain, and a second loss ('yo-yo diet regimen'). We measured the distribution of orally garaged [[sup.14C]]hexachlorobenzene, which is sparingly metabolized. We found that weight cycling has different effects in different organs. Continued weight loss resulted in a threefold increase of [sup.14C] amount and concentration in the brain. After weight regain, [sup.14C] in the brain decreased but then increased again after a second weight loss. Weight loss resulted in an increase in the concentration of [sup.14C] in adipose tissue without changing the total amount in that tissue. Weight loss and regain resulted in an increase of [sup.14C] in the liver, which reflected an increase of fat in the liver. The regimen of weight gain and loss was repeated in mice gavaged with [[sup.14C]]hexachlorobenzene, with one group receiving the nonabsorbable fat olestra in the diet. Combined dietary olestra and caloric restriction caused a 30-fold increase in the rate of excretion of [sup.14C] relative to an ad libitum diet or a reduced caloric diet alone. Distribution of [sup.14C] into the brain resulting from the restricted diet was reduced by 50% by dietary olestra. body weight; xenobiotics; lipophilic; cycling; high fat