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Protein adsorption at interfaces detected by second harmonic generation

Authors :
Salafsky, J.S.
Eisenthal, K.B.
Source :
Journal of Physical Chemistry B. August 17, 2000, Vol. 104 Issue 32, 7752-7755
Publication Year :
2000

Abstract

A method is presented based on second harmonic generation for detection of proteins adsorbed to interfaces and in the presence of bulk species. The free energy of adsorption of cytochrome c to glass was determined from an adsorption isotherm measurement of the second harmonic signal as a function of the bulk protein concentration.

Subjects

Subjects :
Cytochrome c -- Research
Adsorption -- Research
Proteins -- Research
Harmonic functions -- Research
Chemicals, plastics and rubber industries

Details

Language :
English
ISSN :
15206106
Volume :
104
Issue :
32
Database :
Gale General OneFile
Journal :
Journal of Physical Chemistry B
Publication Type :
Academic Journal
Accession number :
edsgcl.124611604

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