Eutectic phase polymerization of activated ribonucleotide mixtures yields quasi-equimolar incorporation of purine and pyrimidine nucleobases

The synthesis of random medium-size ribonucleic acid (RNA) analogues with mixed sequences in ice eutectic phases that are produced when dilute solutions of activated monomers and catalysts are frozen and maintained at -18 degree Celsius for a period of 38 days is described. The results suggest that the eutectic phases in ice are suitable for the random RNA analogue polymerization that will yield pools of medium-size polymers.