Dynein light chain LC8 promotes assembly of the coiled-coil domain of swallow protein

The constructs of swallow, each containing a predicted coiled-coil domain and variable surrounding segments that lie within the domain proposed to interact with LC8, are prepared. By using the glutathione S-transferase (GST) pull-down assays, limited proteolysis followed by mass spectrometry, and circular dichroic spectroscopy, the interaction between LC8 and swallow domains was characterized.