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Simultaneous DNA binding and bending by EcoRV enduclease observed by real-time fluorescence
- Source :
- Biochemistry. Dec 16, 2003, Vol. 42 Issue 49, 14375-14385
- Publication Year :
- 2003
-
Abstract
- The combining fluorescence anisotropy with fluorescence resonance energy transfer (FRET) measures, the complete catalytic cycle of EcoRV endonuclease. Binding, bending and cleavage of substrate oligonucleotide were monitored in real time anisotropy.
Details
- Language :
- English
- ISSN :
- 00062960
- Volume :
- 42
- Issue :
- 49
- Database :
- Gale General OneFile
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.121110759