Integration of Cot analysis, DNA cloning, and high-throughput sequencing facilities genome characterization and gene discovery

Cot based sequence discovery represents a powerful means by which both low-copy and repetitive sequences can be selectively and efficiently divided, cloned, and characterized. Based upon its results, hydroxyapatite chromatography was used to divide sorghum genomic DNA into highly repetitive (HR), moderately repetitive (MR) and single copy (SL) sequence components that were consequently cloned to produce HRCot, MRCot, and SLCot genomic libraries.