Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria

Research analyzes the distribution of human intestinal bifidobacteria in intestinal tracts by a combination of real-time polymerase chain reaction (PCR) with Bifidobacterium genus- and species-specific primers. Results show that the procedure can also be applied to detect Bifidobacterium in feces. Three groups, Bifidobacterium adolescentis group, B. catenulatum group, and B. longum are idetified.