Role of the Bacillus methanolicus citrate synthase II gene, citY, in regulating the secretion of glutamate in L-lysine-secreting mutants

Results indicate that Bacillus methanolicus exhibit several forms of citrae synthase and glutamate secretion can be altered to increase L-lysine secretion by regulaing citrate synthase activity. In the citrate synthase deficient-mutant , the level of of glutamate secreted is reduced 7-fold and the ratio of L-lysine to glutamate secreted is enhance 4-5-fold as compared to the wild type.