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Cigarette smoke alters circRNA expression in human T-cells [version 1; peer review: 1 approved with reservations]

Authors :
So Jin Hong
Zhaohao Liao
Kenneth W. Witwer
Ashley E. Russell
Author Affiliations :
<relatesTo>1</relatesTo>Department of Molecular and Cellular Biology, Krieger School of Arts & Sciences, Johns Hopkins University, Baltimore, Maryland, USA<br /><relatesTo>2</relatesTo>Department of Molecular and Comparative Pathobiology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA<br /><relatesTo>3</relatesTo>Richman Family Precision Medical Center of Excellence in Alzheimer's Disease, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA<br /><relatesTo>4</relatesTo>Department of Neurology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA<br /><relatesTo>5</relatesTo>Department of Biology, School of Science, Penn State Erie The Behrend College, Erie, Pennsylvania, USA
Source :
F1000Research. 12:566
Publication Year :
2023
Publisher :
London, UK: F1000 Research Limited, 2023.

Abstract

Circular RNAs (circRNAs), once thought to be a result of splicing errors, have been found to be involved in various molecular processes and in the pathology of various diseases, including cancer and neurodegenerative diseases. Additionally, circRNA expression was found to be altered by lifestyle habits, such as smoking cigarettes. Past studies have revealed that the rate of smoking remains high in people living with human immunodeficiency virus (HIV). In this study, we isolated total RNA from uninfected T-cells that have been exposed to cigarette smoke and compared the expression levels of circRNAs to those of T-cells that were not exposed to cigarette smoke. We identified certain circRNAs that were upregulated or downregulated in T-cells when exposed to cigarette smoke. These data indicate that the study of circRNAs is warranted within the context of HIV.

Details

ISSN :
20461402
Volume :
12
Database :
F1000Research
Journal :
F1000Research
Notes :
[version 1; peer review: 1 approved with reservations]
Publication Type :
Academic Journal
Accession number :
edsfor.10.12688.f1000research.130430.1
Document Type :
data-paper
Full Text :
https://doi.org/10.12688/f1000research.130430.1