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Sargassum sp. extract improve hematological profile of tilapia fish ( Oreochromis niloticus) [version 3; peer review: 1 approved, 1 approved with reservations]
- Source :
- F1000Research. 12:293
- Publication Year :
- 2024
- Publisher :
- London, UK: F1000 Research Limited, 2024.
-
Abstract
- Background Strategies to increase body resistance and prevent disease in aquaculture include using vaccines, antibiotics, and probiotics. Today, the use of antibiotics with natural ingredients is becoming a trend. Sargassum sp is a natural ingredient that contains high antioxidants and antibiotics. Methods This research was conducted from March to May 2022 at the Biotechnology Laboratory, Faculty of Fisheries and Marine, Universitas Riau, in two stages: 1) the sensitivity of extracts of Sargassum sp. and 2) the application of Sargassum sp. extract orally in tilapia ( O. niloticus). The parameters measured were clear zone, minimum inhibitory concentration, LD 50 test of leaf extract of Sargassum sp. in tilapia ( O. niloticus), hemoglobin levels, hematocrit, total leukocytes, total erythrocytes, leukocyte differentiation, and survival rate. Data on hematology parameters were tabulated and analyzed using a One-Way ANOVA followed by a Student Newman Keuls (SNK) test when deemed necessary. Results The results showed that the extract of Sargassum sp. inhibited the growth of Aeromonas hydrophila bacteria with a clear zone of 6.5-15.0 mm, which is classified as resistant. At doses of 2000, 2500, and 3000 ppm, it did not cause death in fish for 96 hours (LD 50). Hematological parameters can be a sign of the health status of fish. Tilapia given Sargassum sp. in different doses gave an effect between treatments after 30 days of rearing and post-test against A. hydrophila bacteria (pSargassum sp. extract was in the normal or healthy range. Healthy tilapia had erythrocyte counts ranging from 1.34-2.11×10 6 cells/mm 3, hematocrit 26.17-33.19%, hemoglobin 6.26-11.2 g/dL and total leukocytes 1.01-1.50×10 4 cells/mm 3 and total erythrocytes 5.88-9.13×10 4 cells/ mm 3. Conclusions A dose of 3000 ppm provided the highest health improvement against A. hydrophila bacterial infection.
Details
- ISSN :
- 20461402
- Volume :
- 12
- Database :
- F1000Research
- Journal :
- F1000Research
- Notes :
- Revised Amendments from Version 2 Here are some revisions to our manuscript: 1. Abstract: change of sentence "one of the natural ingredients that contain high antioxidant and antibiotics is sargassum sp.” being “Sargassum sp is a natural ingredient that contains high antioxidants and antibiotics” 2. Method: the MIC method adds a sentence “aiming to obtain a minimum dose that can inhibit bacterial growth and is safe to use for fish”. 3. Method: sentence addition “A recirculation system and a filter (dacron) are added to the aquarium. The dacron filter is checked every two days; if dirty, it will be replaced with a new dacron. This aims to maintain water quality, supporting the growth and survival of fish. 4. The addition of the method of observation of leukocyte differentiation. 5. Superscript and caption changes in table 3 and table 4., , [version 3; peer review: 1 approved, 1 approved with reservations]
- Publication Type :
- Academic Journal
- Accession number :
- edsfor.10.12688.f1000research.128819.3
- Document Type :
- research-article
- Full Text :
- https://doi.org/10.12688/f1000research.128819.3