PCR: Methods Express

PCR is the most widely used technique in molecular biology. New PCR variants offering substantial benefits to existing protocols appear on a frequent basis. PCR: Methods Express describes the very latest PCR-based methodologies and approaches to provide the most up-to-date practical advice on how to tackle a broad range of biological problems including: •real time qRT-PCR •rapid generation of gene targeting constructs •PCR multiplexes •PCR-based mutagenesis •identification of microdeletions and microduplications •DNA methylation analysis •forensic genetic DNA typing •genotyping •identification of mutations in single cells •whole genome amplification •diagnosis of infectious diseases •inverse PCR-based RFLP This book is a comprehensive research guide; every chapter discusses the merits and limitations of the available approaches and then provides fully-proven protocols with hints and tips for success. PCR: Methods Express is an essential laboratory manual for researchers in all life science fields and at all levels, from postgraduate student to principal investigator.