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Transcriptomic analysis reveals that mTOR pathway can be modulated in macrophage cells by the presence of cryptococcal cells
- Source :
- Genetics and Molecular Biology, Vol 44, Iss 3 (2021)
- Publication Year :
- 2021
- Publisher :
- Sociedade Brasileira de Genética, 2021.
-
Abstract
- Abstract Cryptococcus neoformans and Cryptococcus gattii are the etiological agents of cryptococcosis, a high mortality disease. The development of such disease depends on the interaction of fungal cells with macrophages, in which they can reside and replicate. In order to dissect the molecular mechanisms by which cryptococcal cells modulate the activity of macrophages, a genome-scale comparative analysis of transcriptional changes in macrophages exposed to Cryptococcus spp. was conducted. Altered expression of nearly 40 genes was detected in macrophages exposed to cryptococcal cells. The major processes were associated with the mTOR pathway, whose associated genes exhibited decreased expression in macrophages incubated with cryptococcal cells. Phosphorylation of p70S6K and GSK-3β was also decreased in macrophages incubated with fungal cells. In this way, Cryptococci presence could drive the modulation of mTOR pathway in macrophages possibly to increase the survival of the pathogen.
- Subjects :
- Macrophage
mTOR pathway
Cryptococcus
RNAseq
interatomic networks
Genetics
QH426-470
Subjects
Details
- Language :
- English
- ISSN :
- 16784685
- Volume :
- 44
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Genetics and Molecular Biology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.ff4ae0253c49768dca8b0bd27a7180
- Document Type :
- article
- Full Text :
- https://doi.org/10.1590/1678-4685-gmb-2020-0390