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Effect of Chitosan Composite Preservative on Proteome Changes of Grass Carp Muscle during Cold Storage

Authors :
LI Jialei, CHEN Sai, TIAN Mingli, YU Jian, LIU Yongle, WANG Faxiang
Source :
Shipin Kexue, Vol 44, Iss 9, Pp 147-153 (2023)
Publication Year :
2023
Publisher :
China Food Publishing Company, 2023.

Abstract

To explore the effect of a chitosan-based preservative solution (10 mg/mL chitosan + 5 mg/mL tea polyphenol + 2 000 U/mL lysozyme) on the change in the muscle proteome of grass carp during cold storage, differential proteomic analysis of fresh fish and nine-day-stored fish with or without (control) preservative treatment was conducted by two-dimensional electrophoresis-mass spectrometry (2DE-MS). The results showed that the 2DE map of the preservative-treated group was not obviously different from that of the fresh group, except for a decrease in the abundance of fast troponin T and an increase in the abundance of slow troponin T and adenylate kinase isoenzyme. However, the 2DE profile of the control group differed greatly from that of the fresh group in both the number and signal intensity of protein spots. Further analysis showed that the abundance of fast troponin T, tropomodulin 4, synaptopodin 2 and pyruvate kinase type M were down-regulated, while the abundance of myosin heavy chain, myosin light chain 1, α-actin, α-tropomyosin and intermediate light meromyosin were up-regulated. Moreover, compared with the preservative-treated group, the abundance of three troponins T were decreased, while the abundance of myosin heavy chain, intermediate light meromyosin, α-actin, α-tropomyosin and creatine kinase M-type were increased in the control group, suggesting that creatine kinase could serve as an indicator for evaluating grass carp muscle spoilage. This study demonstrates that the chitosan-based composite preservative can maintain the stability of grass carp muscle protein during cold storage.

Details

Language :
English, Chinese
ISSN :
10026630
Volume :
44
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Shipin Kexue
Publication Type :
Academic Journal
Accession number :
edsdoj.fce3ed0989f14023800562a33fc9ae48
Document Type :
article
Full Text :
https://doi.org/10.7506/spkx1002-6630-20220331-394