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STIMULATION OF RAPID REGENERATION BY A MAGNETIC FIELD IN PAULOWNIA NODE CULTURES

Authors :
Özge Çelik
Çimen Atak
Aitekin Rzakulieva
Source :
Journal of Central European Agriculture, Vol 9, Iss 2, Pp 297-304 (2008)
Publication Year :
2008
Publisher :
University of Zagreb, Faculty of Agriculture, 2008.

Abstract

In this study, the aim was to determine the effect of magnetic fields on regeneration of Paulownia node cultures. Paulownia tomentosa node cultures were used to generate explants and these explants were passed through a 2.9- 4.6-mT magnetic flux density 1 and 9 times at 2.2 and 19.8 seconds, respectively. Chlorophyll quantities, total RNA concentrations of shoots and shoot formation rates from control and treated explants were determined. While the shoot formation rate was 61.9% in the control group, this rate was increased in magnetic field experiments and shoot formation was 82.5% in the explants that were exposed to a magnetic field for a 2.2 second period. However, the regeneration percentage of the explants exposed to a MF for a period of 19.8 s was 45%. Chlorophyll a, chlorophyll b and total chlorophyll contents of the 2.2 s group were increased in comparison to the control group. Total RNA concentrations of seedlings regenerated from treatment explants treated for 2.2 seconds significantly increased in comparison to the control (p

Details

Language :
Bulgarian, Czech, English, Croatian, Hungarian, Polish, Slovak
ISSN :
13329049
Volume :
9
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Journal of Central European Agriculture
Publication Type :
Academic Journal
Accession number :
edsdoj.fcaead0b51f46e1a9eb85f5a97bfe02
Document Type :
article