Colletotrichum gloeosporioides- Contaminated Tea Infusion Blocks Lipids Reduction and Induces Kidney Damage in Mice

When the homogenate of fresh tea tree leaves was fermented to produce black tea beverage, the Colletotrichum gloeosporioides (main pathogen or endophyte of Camellia sinensis) may be mixed into the fermentation liquor. However, it was unclear whether C. gloeosporioides-contaminated tea beverage would damage human health. Therefore, we investigated the changes of functional components and the influences on mice. C. gloeosporioides was added to the green tea infusion. After cultivation of 48 h, tea polyphenols, caffeine, and L-theanine decreased by 31.0, 26.2, and 8.3%, respectively. The contaminated tea infusion showed brown stain, and produced a group of toxic materials named phthalic acid esters. The animal study showed that green tea without contamination significantly decreased levels of alanine aminotransferase, triglycerides, free fatty acids, low-density lipoprotein, and increased insulin level compared with obese mice. On the contrary, contaminated tea lost the effects on these indicators. Furthermore, the urea nitrogen and serum creatinine levels significantly increased in the contaminated tea-drinking mice. Altogether, our results indicate that C. gloeosporioides contamination can reduce the amount of functional components of green tea. Therefore, it inhibits some health-care function of lipid-lowering. In addition, the toxic components in contaminated tea infusion might induce renal damage.